2005 NASDAQ-100 Open – Women's singles

Kim Clijsters defeated Maria Sharapova in the final, 6–3, 7–5 to win the women's singles tennis title at the 2005 Miami Open. She did not lose a set during the tournament, and became the second woman to achieve the Sunshine Double (after Steffi Graf). At the time, Clijsters was the lowest-ranked player to win the title, doing so as the world No. 38.

Serena Williams was the three-time defending champion, but lost in the quarterfinals to her sister, Venus Williams.

Seeds
All seeds received a bye into the second round. • # 🇫🇷 Amélie Mauresmo (semifinals)

• # 🇷🇺 Maria Sharapova (final)

• # 🇺🇸 Serena Williams (quarterfinals)

• # 🇷🇺 Elena Dementieva (quarterfinals)

• # 🇷🇺 Anastasia Myskina (fourth round)

• # 🇷🇺 Svetlana Kuznetsova (fourth round)

• # 🇦🇺 Alicia Molik (fourth round)

• # 🇺🇸 Venus Williams (semifinals)

• # 🇷🇺 Vera Zvonareva (second round)

• # 🇷🇺 Nadia Petrova (second round)

• # Patty Schnyder (third round)

• # 🇫🇷 Nathalie Dechy (third round)

• # 🇷🇺 Elena Bovina (third round)

• # 🇮🇹 Francesca Schiavone (third round)

• # 🇷🇺 Elena Likhovtseva (fourth round)

• # Karolina Šprem (fourth round)

• # 🇦🇷 Paola Suárez (second round)

• # Jelena Janković (second round)

• # 🇧🇪 Justine Henin-Hardenne (quarterfinals)

• # 🇸🇰 Daniela Hantuchová (second round)

• # 🇯🇵 Ai Sugiyama (third round)

• # 🇫🇷 Tatiana Golovin (fourth round)

• # 🇯🇵 Shinobu Asagoe (fourth round)

• # 🇺🇸 Amy Frazier (second round)

• # 🇨🇴 Fabiola Zuluaga (third round)

• # 🇮🇹 Flavia Pennetta (third round)

• # 🇧🇬 Magdalena Maleeva (third round)

• # 🇫🇷 Marion Bartoli (second round)

• # 🇦🇷 Gisela Dulko (third round)

• # 🇮🇱 Anna Smashnova (third round)

• # 🇷🇺 Dinara Safina (second round)

• # 🇨🇿 Klára Koukalová (second round)

Seeds
• # 🇺🇦 Yuliana Fedak (qualified)

• # 🇺🇸 Laura Granville (qualified)

• # 🇺🇦 Alona Bondarenko (qualified)

• # 🇫🇷 Séverine Beltrame (first round)

• # 🇦🇷 Mariana Díaz Oliva (qualified)

• # 🇫🇷 Stéphanie Cohen-Aloro (qualifying competition, Lucky loser)''

• # 🇱🇺 Anne Kremer (first round)

• # 🇫🇷 Stéphanie Foretz (qualified)

• # 🇮🇹 Antonella Serra Zanetti (first round)

• # 🇺🇸 Lilia Osterloh (first round)

• # 🇯🇵 Aiko Nakamura (qualifying competition)

• # 🇭🇺 Petra Mandula (moved to Main Draw)

• # Sanda Mamić (first round)

• # Silvija Talaja (qualifying competition)

• # 🇨🇿 Michaela Paštiková (first round)

• # Emmanuelle Gagliardi (qualifying competition)

• # 🇦🇺 Evie Dominikovic (qualifying competition)

• # 🇨🇴 Catalina Castaño (qualified)

• # 🇸🇰 Ľubomíra Kurhajcová (first round)

• # 🇫🇷 Camille Pin (first round)

• # 🇰🇷 Cho Yoon-jeong (first round)

• # 🇹🇳 Selima Sfar (first round)

• # 🇩🇪 Julia Schruff (qualified)

• # 🇮🇱 Tzipora Obziler (first round)

• # 🇬🇧 Elena Baltacha (qualifying competition)

Qualifiers
• # 🇺🇦 Yuliana Fedak

• # 🇺🇸 Laura Granville

• # 🇺🇦 Alona Bondarenko

• # 🇩🇪 Julia Schruff

• # 🇦🇷 Mariana Díaz Oliva

• # 🇪🇸 Magüi Serna

• # 🇺🇸 Jewel Peterson

• # 🇫🇷 Stéphanie Foretz

• # 🇮🇱 Shahar Pe'er

• # 🇨🇴 Catalina Castaño

• # 🇷🇺 Galina Voskoboeva

• # 🇩🇪 Sandra Klösel

Lucky loser

 * 1) 🇫🇷 Stéphanie Cohen-Aloro