2005 Zentiva Czech Indoor Open – Women's singles

The 2005 Zentiva Czech Indoor Open was a women's tennis tournament played on indoor hard courts in Průhonice, Czech Republic that was part of the 2005 ITF Women's Circuit. It was the first edition of the tournament and was held from 14 to 20 November 2005.

Lucie Hradecká won the title, defeating Agnieszka Radwańska in the final, 4–6, 6–1, 7–6(10–8).

Seeds
• # Ivana Lisjak (first round)

• # 🇦🇹 Yvonne Meusburger Garamszegi(second round)

• # 🇨🇿 Michaela Paštiková (quarterfinals)

• # 🇨🇿 Barbora Strýcová(second round)

• # 🇨🇿 Libuše Průšová (second round)

• # 🇩🇪 Kathrin Wörle-Scheller (quarterfinals)

• # 🇨🇿 Sandra Kleinová (second round)

• # 🇨🇿 Olga Vymetálková (second round)