2006 Italian Open – Women's singles

Martina Hingis defeated Dinara Safina in the final, 6–2, 7–5 to win the women's singles tennis title at the 2006 Italian Open. It was her first title since coming out of retirement.

Amélie Mauresmo was the reigning champion, but withdrew due to illness.

Seeds
The top eight seeds received a bye into the second round. Champion seeds are shown in bold while text in italics indicates the round in which seeds were eliminated. • # 🇫🇷 Amélie Mauresmo (withdrew due to illness)

• # 🇧🇪 Kim Clijsters (third round)

• # 🇷🇺 Maria Sharapova (withdrew due to right foot)

• # 🇷🇺 Nadia Petrova (withdrew due to right pectoralis strain)

• # 🇷🇺 Elena Dementieva (quarterfinals)

• # Patty Schnyder (third round)

• # 🇷🇺 Svetlana Kuznetsova (semifinals)

• # 🇮🇹 Francesca Schiavone (third round)

• # 🇺🇸 Venus Williams (semifinals)

• # 🇷🇺 Anastasia Myskina (third round)

• # 🇩🇪 Anna-Lena Grönefeld (third round)

• # 🇨🇿 Nicole Vaidišová (second round)

• # 🇸🇰 Daniela Hantuchová (first round)

• # 🇷🇺 Elena Likhovtseva (first round)

• # 🇮🇹 Flavia Pennetta (quarterfinals)

• # 🇷🇺 Dinara Safina (final)

• # 🇫🇷 Nathalie Dechy (second round)

• # 🇯🇵 Ai Sugiyama (second round)