2007 Italian Open – Women's singles

Jelena Janković defeated Svetlana Kuznetsova in the final, 7–5, 6–1 to win the women's singles tennis title at the 2007 Italian Open. It was her second Tier I title.

Martina Hingis was the reigning champion, but did not participate this year due to a hip injury.

Seeds
The top eight seeds received a bye into the second round. • # 🇫🇷 Amélie Mauresmo (second round)

• # 🇷🇺 Svetlana Kuznetsova (finals)

• # 🇷🇸 Jelena Janković (champion)

• # 🇨🇿 Nicole Vaidišová (withdrew due to a right wrist injury)

• # 🇷🇺 Nadia Petrova (third round; retired due to low back pain)

• # 🇷🇺 Dinara Safina (quarterfinals)

• # 🇷🇺 Anna Chakvetadze (third round)

• # 🇺🇸 Serena Williams (quarterfinals)

• # 🇸🇰 Daniela Hantuchová (semifinals)

• # 🇷🇺 Elena Dementieva (quarterfinals)

• # 🇮🇱 Shahar Pe'er (third round)

• # 🇷🇸 Ana Ivanovic (withdrew due to an ankle injury)

• # 🇨🇳 Li Na (withdrew due to gastrointestinal illness)

• # Patty Schnyder (semifinals)

• # Katarina Srebotnik (second round)

• # 🇮🇹 Tathiana Garbin (second round)