2009 Australian Open – Girls' singles

Ksenia Pervak won the title, defeating Laura Robson in the final 6–3, 6–1.

Arantxa Rus was the defending champion, but was no longer eligible to participate in junior events. She competed in the women's singles qualifying competition where she lost to Julia Schruff in the second round.

Seeds
• # 🇹🇭 Noppawan Lertcheewakarn (semifinals)

• # 🇷🇴 Ana Bogdan (semifinals)

• # 🇷🇺 Ksenia Pervak (champion)

• # 🇷🇴 Elena Bogdan (quarterfinals)

• # 🇬🇧 Laura Robson (final)

• # Ajla Tomljanović (first round)

• # 🇫🇷 Kristina Mladenovic (quarterfinals)

• # 🇺🇸 Lauren Embree (third round)

• # 🇬🇧 Heather Watson (quarterfinals)

• # 🇯🇵 Aki Yamasoto (second round)

• # 🇰🇿 Zarina Diyas (first round)

• # 🇩🇪 Linda Berlinecke (first round)

• # 🇷🇺 Yana Buchina (third round)

• # 🇷🇺 Ksenia Kirillova (third round)

• # Beatrice Gumulya (third round)

• # 🇺🇦 Nadiya Kichenok (first round)