2009 Australian Open – Women's singles

Serena Williams defeated Dinara Safina in the final, 6–0, 6–3 to win the women's singles tennis title at the 2009 Australian Open. It was her fourth Australian Open singles title and tenth major singles title overall. With the win, Williams regained the world No. 1 ranking.

Maria Sharapova was the reigning champion, but withdrew from the tournament due to a recurring shoulder injury.

This was also the first Australian Open to feature three Russian semifinalists, those being Safina, Vera Zvonareva and Elena Dementieva.

Seeds
• 🇷🇸 Jelena Janković (fourth round)

• 🇺🇸 Serena Williams (champion)

• 🇷🇺 Dinara Safina (final)

• 🇷🇺 Elena Dementieva (semifinals)

• 🇷🇸 Ana Ivanovic (third round)

• 🇺🇸 Venus Williams (second round)

• 🇷🇺 Vera Zvonareva (semifinals)

• 🇷🇺 Svetlana Kuznetsova (quarterfinals)

• 🇵🇱 Agnieszka Radwańska (first round)

• 🇷🇺 Nadia Petrova (fourth round)

• Caroline Wozniacki (third round)

• 🇮🇹 Flavia Pennetta (third round)

• 🇧🇾 Victoria Azarenka (fourth round, retired due to dizziness and gastrointestinal illness)

•  Patty Schnyder (second round)

• 🇫🇷 Alizé Cornet (fourth round)

• 🇫🇷 Marion Bartoli (quarterfinals)

• 🇷🇺 Anna Chakvetadze (second round)

• 🇸🇰 Dominika Cibulková (fourth round)

• 🇸🇰 Daniela Hantuchová (third round)

• 🇫🇷 Amélie Mauresmo (third round)

• 🇪🇸 Anabel Medina Garrigues (fourth round)

• 🇨🇳 Zheng Jie (fourth round, retired due to a left wrist sprain)

• 🇭🇺 Ágnes Szávay (first round)

• 🇦🇹 Sybille Bammer (first round)

• 🇪🇪 Kaia Kanepi (third round)

• 🇯🇵 Ai Sugiyama (third round)

• 🇷🇺 Maria Kirilenko (first round)

• 🇮🇹 Francesca Schiavone (first round)

• 🇷🇺 Alisa Kleybanova (fourth round)

• 🇨🇦 Aleksandra Wozniak (first round)

• 🇺🇦 Alona Bondarenko (third round)

• 🇹🇭 Tamarine Tanasugarn (first round)