2009 Bancolombia Open – Singles

Marcos Daniel was the defending champion, but he decided to not defend his 2009 title. Horacio Zeballos defeated Santiago González in the final (7–6(7–3), 6–0).

Seeds
• # 🇪🇸 Óscar Hernández (first round)

• # Frederico Gil (second round)

• # 🇦🇷 Brian Dabul (first round)

• # 🇪🇸 Iván Navarro (first round)

• # 🇦🇷 Máximo González (second round)

• # 🇦🇷 Leonardo Mayer (first round, retired)

• # 🇦🇷 Sergio Roitman (first round)

• # 🇧🇷 Thiago Alves (second round)