2009 Copa Sony Ericsson Colsanitas – Singles

Nuria Llagostera Vives was the defending champion, but lost in the second round to Patricia Mayr.

Seeds
• # 🇮🇹 Flavia Pennetta (first round)

• # 🇪🇸 Carla Suárez Navarro (second round)

• # 🇦🇷 Gisela Dulko (final)

• # 🇪🇸 Nuria Llagostera Vives (second round)

• # 🇨🇿 Klára Zakopalová (second round)

• # 🇫🇷 Mathilde Johansson (quarterfinals)

• # 🇪🇸 María José Martínez Sánchez (champion)

• # 🇪🇸 Lourdes Domínguez Lino (first round)