2009 Estoril Open – Women's singles

Maria Kirilenko was the defending champion, but lost in the quarterfinals to Ekaterina Makarova.

Yanina Wickmayer defeated Ekaterina Makarova in the final, 7–5, 6–2. It was Wickmayer's first career title.

Seeds
• # 🇨🇿 Iveta Benešová (second round)

• # 🇷🇺 Maria Kirilenko (quarterfinals)

• # 🇷🇴 Sorana Cîrstea (quarterfinals)

• # 🇩🇪 Sabine Lisicki (quarterfinals, retired)

• # 🇨🇿 Petra Kvitová (first round)

• # 🇷🇺 Ekaterina Makarova (final)

• # 🇮🇱 Shahar Pe'er (semifinals, retired due to a foot injury)

• # 🇩🇪 Anna-Lena Grönefeld (semifinals)