2009 Fifth Third Bank Tennis Championships – Women's singles

This was the 13th edition of the tournament, which was part of the 2009 ITF Women's Circuit, a tier below the 2009 WTA Tour. It was held in Lexington, Kentucky, United States from 20 to 26 July 2009 on outdoor hardcourts. The prize money for women was US$50,000.

Melanie Oudin, the defending champion, declined to participate that year.

Sania Mirza won in the final, defeating top seed Julie Coin 7-6(5), 6-4.

Seeds
• # 🇫🇷 Julie Coin (final)

• # 🇮🇳 Sania Mirza (champion)

• # 🇬🇧 Melanie South (first round)

• # 🇺🇸 Madison Brengle (quarterfinals)

• # 🇨🇦 Valérie Tétreault (second round)

• # 🇨🇳 Yuan Meng (semifinals)

• # 🇫🇷 Yulia Fedossova (second round)

• # 🇯🇵 Aiko Nakamura (first round)