2009 Open Barletta – Città della Disfida – Singles

Mikhail Kukushkin was the defending champion; however, he didn't take part in these championships this year.

Ivo Minář defeated Santiago Ventura in the final (6–4, 6–3).

Seeds
• # 🇪🇸 Daniel Gimeno-Traver (first round)

• # 🇨🇿 Ivo Minář (champion)

• # 🇺🇿 Denis Istomin (semifinals)

• # 🇧🇷 Marcos Daniel (first round)

• # 🇪🇸 Rubén Ramírez Hidalgo (quarterfinals)

• # 🇪🇸 Santiago Ventura (final)

• # Pablo Cuevas (first round)

• # 🇵🇪 Luis Horna (quarterfinals)