2009 Tallahassee Tennis Challenger – Singles

Bobby Reynolds was the defender of championship title; however, he lost to Ryan Sweeting in the first round.

John Isner won in the final 7–5, 6–4, against Donald Young.

Seeds
• # 🇺🇸 Bobby Reynolds (first round)

• # 🇺🇸 Robert Kendrick (semifinals)

• # 🇺🇸 Kevin Kim (second round)

• # 🇺🇸 Vince Spadea (second round)

• # 🇨🇦 Frank Dancevic (first round)

• # 🇯🇵 Go Soeda (second round)

• # 🇺🇸 John Isner (champion)

• # 🇺🇸 Jesse Levine (first round)