2009 UniCredit Czech Open – Singles

The defending champion Agustín Calleri was eliminated in the first round.

Jan Hájek, the 2006 singles champion, came from the qualifying round to beat in the final Belgian player Steve Darcis by 6–2, 1–6, 6–4.

Seeds
• # 🇨🇿 Radek Štěpánek (first round)

• # 🇨🇿 Tomáš Berdych (semifinals)

• # 🇷🇺 Igor Andreev (withdrew)

• # Ivan Ljubičić (quarterfinals)

• # Frederico Gil (first round)

• # 🇨🇿 Ivo Minář (second round)

• # 🇰🇿 Andrey Golubev (first round)

• # 🇪🇸 Iván Navarro (quarterfinals)