2010 Australian Open – Girls' singles

Karolína Plíšková won the title, defeating Laura Robson in the final, 6–1, 7–6(7–5).

Ksenia Pervak was the defending champion, but was no longer eligible to participate in junior events. She competed in the women's singles qualifying competition as the 17th seed, losing to second seed Regina Kulikova in the final round.

Seeds
• # 🇭🇺 Tímea Babos (quarterfinals)

• # 🇬🇧 Heather Watson (first round)

• # 🇷🇺 Daria Gavrilova (quarterfinals)

• # 🇨🇦 Gabriela Dabrowski (second round)

• # Silvia Njirić (quarterfinals)

• # 🇨🇿 Karolína Plíšková (champion)

• # Nastja Kolar (second round)

• # Camila Silva (first round)

• # 🇯🇵 Sachie Ishizu (second round)

• # 🇺🇦 Maryna Zanevska (third round)

• # 🇷🇺 Yulia Putintseva (second round)

• # 🇸🇰 Jana Čepelová (second round)

• # 🇷🇸 Tamara Čurović (first round)

• # 🇳🇴 Ulrikke Eikeri (first round)

• # 🇨🇳 Zheng Saisai (first round)

• # 🇷🇺 Yana Buchina (third round, retired)