2010 Challenger Ciudad de Guayaquil – Singles

Nicolás Lapentti was the defending champion; however, he retired from professional tennis before this tournament. Paul Capdeville won the title, defeating Diego Junqueira 6–3, 3–6, 6–3 in the final.

Seeds
• # 🇦🇷 Carlos Berlocq (semifinals)

• # 🇦🇷 Brian Dabul (first round)

• # 🇧🇷 João Souza (second round)

• # 🇦🇷 Horacio Zeballos (first round)

• # Grega Žemlja (second round)

• # 🇧🇷 Marcos Daniel (quarterfinals, retired)

• # 🇦🇷 Máximo González (semifinals)

• # 🇫🇷 Éric Prodon (quarterfinals)