2010 Fifth Third Bank Tennis Championships – Singles

Harel Levy was the winner in 2009, but he chose to not participate this year.

Carsten Ball became the new champion, after he won 6–4, 7–6(2), against Jesse Levine.

Seeds
• # 🇦🇺 Carsten Ball (champion)

• # 🇱🇹 Ričardas Berankis (second round, retired due to tricep strain)

• # 🇧🇷 João Souza (first round)

• # 🇺🇸 Kevin Kim (quarterfinals)

• # 🇺🇸 Jesse Levine (final)

• # Paul Capdeville (quarterfinals)

• # 🇨🇦 Peter Polansky (semifinals)

• # 🇦🇺 Greg Jones (first round)