2010 Knoxville Challenger – Singles

Taylor Dent was the defending champion, however he chose to not compete this year.

Kei Nishikori won this tournament. He defeated Robert Kendrick 6–1, 6–4 in the final.

Seeds
• # 🇺🇸 Ryan Sweeting (first round)

• # 🇺🇸 Donald Young (first round)

• # 🇯🇵 Kei Nishikori (champion)

• # 🇦🇺 Peter Luczak (first round)

• # 🇦🇺 Marinko Matosevic (withdrew)

• # 🇺🇸 Robert Kendrick (final)

• # 🇦🇺 Carsten Ball (second round)

• # 🇺🇸 Tim Smyczek (first round)