2010 Sarasota Open – Singles

James Ward was the defending champion, but he lost against Víctor Estrella in the first round. Kei Nishikori, who played in this tournament as a 'special exempt', won in the final 2–6, 6–3, 6–4, against Brian Dabul.

Seeds
• # 🇺🇸 Taylor Dent (first round, retired)

• # 🇺🇸 Jesse Levine (quarterfinals)

• # 🇦🇺 Carsten Ball (withdrew due to Davis Cup)

• # 🇦🇷 Brian Dabul (final)

• # 🇺🇸 Kevin Kim (second round)

• # 🇺🇸 Ryan Sweeting (semifinals)

• # 🇺🇸 Donald Young (first round)

• # 🇺🇸 Alex Kuznetsov (second round)

• # 🇺🇸 Robert Kendrick (first round)