2010 Sparta Prague Open – Singles

Number one seed Lucie Hradecká defeated unseeded Ajla Tomljanović 6–1, 7–6(7–4) in the final to win the women's singles title at the inaugural Sparta Prague Open.

Seeds
• # 🇨🇿 Lucie Hradecká (champion)

• # 🇨🇿 Sandra Záhlavová (quarterfinals)

• # 🇦🇺 Jelena Dokić (quarterfinals)

• # Maša Zec Peškirič (quarterfinals)

• # 🇷🇺 Ksenia Pervak (semifinals)

• # 🇧🇾 Darya Kustova (second round)

• # 🇮🇹 Anna Floris (first round)

• # 🇨🇿 Andrea Hlaváčková (first round)