2010 Tallahassee Tennis Challenger – Singles

John Isner was the defending of champion, but he chose not to participate this year. Brian Dabul won in the final, after Robby Ginepri retired at the score of 6–4, 0–4.

Seeds
• # 🇺🇸 Rajeev Ram (semifinals)

• # Kevin Anderson (withdrew due to tendonitis)

• # 🇺🇸 Taylor Dent (first round)

• # 🇺🇸 Robby Ginepri (final, retired)

• # 🇺🇸 Jesse Levine (first round)

• # 🇦🇺 Carsten Ball (quarterfinals)

• # 🇺🇸 Kevin Kim (first round)

• # 🇺🇸 Ryan Sweeting (quarterfinals)