2010 Western & Southern Financial Group Women's Open – Singles

Kim Clijsters defeated Maria Sharapova in the final, 2–6, 7–6(7–4), 6–2 to win the women's singles tennis title at the 2010 Cincinnati Masters. She saved three championship points en route to the title. It was Sharapova's second final defeat in as many weeks, having lost the Stanford final to Victoria Azarenka just two weeks earlier.

Jelena Janković was the defending champion, but lost in the third round to Akgul Amanmuradova.

Ana Ivanovic, ranked as the world No. 62 entering the tournament, upset Azarenka in the first round, having trailed 2–6, 2–5 before fighting back to win the match, 2–6, 7–6(8–6), 6–2. She then went on to make her third semifinal of the year, being forced to retire against Clijsters after injuring her foot.

Seeds
The top eight seeds receive a bye into the second round. • # 🇷🇸 Jelena Janković (third round)

• # Caroline Wozniacki (third round)

• # 🇷🇺 Elena Dementieva (second round)

• # 🇧🇪 Kim Clijsters (champion)

• # 🇮🇹 Francesca Schiavone (second round)

• # 🇷🇺 Vera Zvonareva (third round)

• # 🇵🇱 Agnieszka Radwańska (third round)

• # 🇨🇳 Li Na (third round)

• # 🇧🇾 Victoria Azarenka (first round)

• # 🇷🇺 Maria Sharapova (final)

• # 🇮🇹 Flavia Pennetta (quarterfinals)

• # 🇧🇪 Yanina Wickmayer (quarterfinals)

• # 🇮🇱 Shahar Pe'er (third round)

• # 🇫🇷 Aravane Rezaï (first round)

• # 🇷🇺 Nadia Petrova (first round, retired due to heat illness)

• # 🇫🇷 Marion Bartoli (quarterfinals)