2012 Knoxville Challenger – Singles

Jesse Levine was the defending champion but was defeated in the First Round by Tennys Sandgren. Michael Russell won the final 6–3, 6–2 against Bobby Reynolds.

Seeds
• # 🇺🇸 Jesse Levine (first round)

• # 🇷🇺 Alex Bogomolov Jr. (first round, retired because of a shoulder injury)

• # 🇺🇸 Michael Russell (champion)

• # 🇩🇪 Mischa Zverev (second round)

• # 🇺🇸 Ryan Sweeting (second round)

• # 🇺🇸 Bobby Reynolds (final)

• # 🇺🇸 Tim Smyczek (semifinals)

• # 🇺🇸 Denis Kudla (quarterfinals)