2016 Aegon International Eastbourne – Singles

Belinda Bencic was the defending champion, but lost in the second round to Elena Vesnina.

Dominika Cibulková won the title, defeating Karolína Plíšková in the final, 7–5, 6–3.

Seeds
All seeds received a bye into the second round. • 🇵🇱 Agnieszka Radwańska (quarterfinals)

• 🇮🇹 Roberta Vinci (second round)

• Belinda Bencic (second round)

• Timea Bacsinszky (second round)

• 🇨🇿 Petra Kvitová (third round)

• 🇷🇺 Svetlana Kuznetsova (second round)

• 🇦🇺 Samantha Stosur (second round)

• 🇪🇸 Carla Suárez Navarro (second round)

• 🇺🇸 Madison Keys (withdrew)

• 🇨🇿 Karolína Plíšková (final)

• 🇬🇧 Johanna Konta (semifinals)

• 🇸🇰 Dominika Cibulková (champion)

• 🇮🇹 Sara Errani (second round)

• 🇷🇺 Anastasia Pavlyuchenkova (second round)

• 🇷🇴 Irina-Camelia Begu (second round)

• 🇨🇿 Lucie Šafářová (second round)

Seeds
• # Monica Puig (qualified)

• # 🇲🇪 Danka Kovinić (first round)

• # Mirjana Lučić-Baroni (qualified)

• # 🇺🇸 Madison Brengle (qualified)

• # 🇧🇪 Kirsten Flipkens (withdrew, still competing in Mallorca)

• # 🇨🇳 Zhang Shuai (qualifying competition, lucky loser)

• # 🇺🇸 Varvara Lepchenko (qualified)

• # 🇺🇦 Kateryna Bondarenko (qualified)

• # 🇺🇸 Christina McHale (withdrew)

• # 🇺🇸 Irina Falconi (first round)

• # 🇺🇸 Louisa Chirico (first round)

• # 🇺🇸 Nicole Gibbs (first round)

• # 🇹🇷 Çağla Büyükakçay (first round)

• # 🇨🇳 Zheng Saisai (qualifying competition, lucky loser)

• # 🇨🇴 Mariana Duque Mariño (first round)

• # 🇨🇿 Denisa Allertová (qualifying competition, lucky loser)

Qualifiers
• # Monica Puig

• # Polona Hercog

• # Mirjana Lučić-Baroni

• # 🇺🇸 Madison Brengle

• # 🇧🇪 Alison Van Uytvanck

• # Ana Konjuh

• # 🇺🇸 Varvara Lepchenko

• # 🇺🇦 Kateryna Bondarenko

Lucky losers
• # 🇨🇳 Zhang Shuai

• # 🇨🇳 Zheng Saisai

• # 🇨🇿 Denisa Allertová

• # 🇪🇪 Anett Kontaveit