2016 Australian Open – Girls' singles

Vera Lapko won the title, defeating defending champion Tereza Mihalíková in the final, 6–3, 6–4.

Seeds
• 🇨🇦 Bianca Andreescu (third round; withdrew)

• 🇸🇰 Tereza Mihalíková (final)

• 🇨🇦 Charlotte Robillard-Millette (first round)

• 🇷🇺 Anna Kalinskaya (quarterfinals)

• 🇧🇾 Vera Lapko (champion)

• 🇨🇳 Zheng Wushuang (first round)

• 🇦🇺 Kimberly Birrell (withdrew)

• 🇷🇺 Anastasia Potapova (quarterfinals)

• Rebeka Masarova (semifinals)

• 🇮🇳 Pranjala Yadlapalli (third round)

• 🇦🇺 Priscilla Hon (withdrew)

• 🇯🇵 Chihiro Muramatsu (third round)

• 🇯🇵 Mai Hontama (first round)

• 🇨🇿 Lucie Kaňková (second round)

• 🇨🇦 Katherine Sebov (third round)

• 🇺🇦 Dayana Yastremska (quarterfinals)

• 🇺🇦 Anastasia Zarytska (third round)

Seeds
• # 🇷🇸 Olga Danilović (qualified)

• # 🇨🇳 Ma Shuyue (qualifying competition)

• # 🇮🇹 Lucrezia Stefanini (qualified)

• # 🇵🇱 Daria Kuczer (first round)

• # 🇺🇸 Helen Abigail Altick (qualifying competition)

• # Rifanty Kahfiani (first round)

• # Lea Bošković (qualified)

• # 🇯🇵 Ayumi Miyamoto (qualified)

• # Isolde de Jong (first round)

• # Cho I-hsuan (qualified)

• # 🇨🇳 Ni Ma Zhuoma (qualifying competition)

• # 🇯🇵 Naho Sato (first round)

• # Phillis Vanenburg (qualifying competition)

• # 🇯🇵 Misaki Matsuda (first round)

• # 🇮🇪 Georgia Drummy (qualified)

• # 🇨🇳 Mu Shouna (first round)

Qualifiers
• # 🇷🇸 Olga Danilović

• # Cho I-hsuan

• # 🇮🇹 Lucrezia Stefanini

• # 🇯🇵 Ayano Shimizu

• # 🇮🇪 Georgia Drummy

• # 🇦🇺 Sara Tomic

• # Lea Bošković

• # 🇯🇵 Ayumi Miyamoto

Lucky losers
• # 🇯🇵 Satoko Sueno

• # 🇦🇺 Olivia Gadecki