2016 Cal Poly Mustangs football team

The 2016 Cal Poly Mustangs football team represented California Polytechnic State University in the 2016 NCAA Division I FCS football season. The Mustangs were led by eighth-year head coach Tim Walsh and played their home games at Alex G. Spanos Stadium. They were members of the Big Sky Conference. They finished the season 7–5, 5–3 in Big Sky play to finish in a tie for fourth place. They received an at-large bid to the FCS Playoffs where they lost to San Diego in the first round.