2016 Challenger Banque Nationale de Gatineau – Women's singles

Alexa Glatch was the defending champion, but decided not to participate this year.

Bianca Andreescu won the title, defeating Ellie Halbauer 6–2, 7–5 in the final.

Seeds
• #🇺🇸 Jennifer Brady (withdrew)

• #🇨🇿 Barbora Štefková (quarterfinals)

• #🇨🇦 Françoise Abanda (first round)

• #🇺🇸 Lauren Albanese (quarterfinals)

• #🇲🇽 Victoria Rodríguez (first round)

• #🇬🇧 Katy Dunne (second round)

• #🇺🇸 Bernarda Pera (first round)

• #🇺🇸 Francesca Di Lorenzo (first round)