2016 Challenger Ciudad de Guayaquil – Singles

Gastão Elias was the defending champion but lost in the first round to Guilherme Clezar.

Nicolás Kicker won the title after defeating Arthur De Greef 6–3, 6–2 in the final.

Seeds
• # Gastão Elias (first round)

• # 🇦🇷 Facundo Bagnis (quarterfinals)

• # 🇦🇷 Renzo Olivo (withdrew)

• # 🇦🇷 Carlos Berlocq (first round, retired)

• # 🇩🇴 Víctor Estrella Burgos (first round)

• # 🇨🇴 Santiago Giraldo (first round)

• # 🇧🇷 Rogério Dutra Silva (first round)

• # 🇧🇷 João Souza (first round)

• # 🇺🇸 Ernesto Escobedo (second round)