2016 Citi Open – Women's singles

Sloane Stephens was the defending champion, but lost in the first round to Risa Ozaki.

Yanina Wickmayer won the title, defeating Lauren Davis in the final, 6–4, 6–2.

Seeds
• #🇦🇺 Samantha Stosur (quarterfinals)

• #🇺🇸 Sloane Stephens (first round)

• # Monica Puig (second round)

• #🇫🇷 Kristina Mladenovic (quarterfinals)

• #🇨🇦 Eugenie Bouchard (first round)

• #🇰🇿 Yulia Putintseva (semifinals)

• #🇧🇪 Yanina Wickmayer (champion)

• #🇷🇴 Monica Niculescu (first round)

Seeds
• # 🇨🇳 Zhu Lin (qualified)

• # 🇯🇵 Hiroko Kuwata (qualifying competition, lucky loser)

• # 🇷🇺 Alla Kudryavtseva (qualified)

• # 🇯🇵 Miyu Kato (first round)

• # 🇷🇺 Ksenia Lykina (first round)

• # 🇨🇳 Xu Shilin (qualifying competition)

• # 🇨🇳 Yang Zhaoxuan (first round)

• # 🇯🇵 Shuko Aoyama (qualifying competition)

Qualifiers
• # 🇨🇳 Zhu Lin

• # 🇷🇺 Varvara Flink

• # 🇷🇺 Alla Kudryavtseva

• # 🇺🇸 Lauren Albanese

Lucky losers

 * 1) 🇯🇵 Hiroko Kuwata