2016 Columbus Challenger 1 – Singles

Dennis Novikov was the defending champion but lost in the first round to Evan King.

Mikael Torpegaard won the title after defeating Benjamin Becker 6–4, 1–6, 6–2 in the final.

Seeds
• # 🇩🇪 Benjamin Becker (final)

• # 🇺🇸 Dennis Novikov (first round)

• # 🇺🇸 Austin Krajicek (first round)

• # 🇨🇦 Peter Polansky (second round)

• # 🇺🇸 Ernesto Escobedo (first round, retired)

• # Marcelo Arévalo (first round)

• # Blaž Rola (second round)

• # 🇧🇷 Guilherme Clezar (first round)