2016 FINA World Swimming Championships (25 m) – Women's 100 metre backstroke

The Women's 100 metre backstroke competition of the 2016 FINA World Swimming Championships (25 m) was held on 6 and 7 December 2016.

Records
Prior to the competition, the existing world and championship records were as follows.

Heats
The heats were held at 12:37.

Semifinals
The semifinals were held at 19:44.

Final
The final was held at 20:01.