2016 French Open – Girls' singles

Rebeka Masarova won the title, defeating Amanda Anisimova in the final, 7–5, 7–5.

Paula Badosa was the defending champion, but was no longer eligible to participate in junior events.

Seeds
• # 🇷🇺 Olesya Pervushina (semifinals)

• # 🇺🇸 Amanda Anisimova (final)

• # 🇺🇸 Kayla Day (third round)

• # 🇷🇺 Anastasia Potapova (semifinals)

• # 🇺🇦 Dayana Yastremska (third round)

• # 🇬🇧 Katie Swan (first round)

• # 🇷🇴 Georgia Crăciun (first round)

• # 🇷🇺 Elena Rybakina (second round)

• # 🇺🇸 Usue Maitane Arconada (third round)

• # 🇺🇸 Sofia Kenin (third round)

• # 🇷🇺 Sofya Zhuk (first round)

• # Rebeka Masarova (champion)

• # 🇨🇿 Markéta Vondroušová (third round)

• # 🇫🇷 Tessah Andrianjafitrimo (second round)

• # 🇭🇺 Panna Udvardy (first round)

• # 🇷🇺 Amina Anshba (first round)

Seeds
• # Jodie Anna Burrage (qualifying competition)

• # Ioana Mincă (qualified)

• # 🇷🇺 Varvara Gracheva (qualified)

• # 🇵🇱 Iga Świątek (qualified)

• # 🇦🇷 María Lourdes Carlé (first round)

• # 🇺🇸 Natasha Subhash (first round)

• # Ylena In-Albon (qualified)

• # 🇧🇪 Lara Salden (first round)

• # 🇩🇪 Irina Cantos Siemers (first round)

• # 🇺🇸 Ellie Douglas (first round)

• # 🇨🇿 Johana Marková (qualifying competition)

• # 🇨🇳 Ren Jiaqi (qualifying competition)

• # 🇪🇸 Paula Arias Manjón (qualified)

• # 🇯🇵 Mayuka Aikawa (qualifying competition)

• # 🇮🇹 Lucrezia Stefanini (qualified)

• # 🇨🇳 Cao Siqi (first round)

Qualifiers
• # 🇮🇹 Lucrezia Stefanini

• # Ioana Mincă

• # 🇷🇺 Varvara Gracheva

• # 🇵🇱 Iga Świątek

• # 🇪🇸 Paula Arias Manjón

• # Eleni Christofi

• # Ylena In-Albon

• # 🇮🇹 Federica Bilardo