2016 IAAF World U20 Championships – Women's 400 metres

The women's 400 metres event at the 2016 IAAF World U20 Championships was held at Zdzisław Krzyszkowiak Stadium on 19, 20 and 21 July.

Heats
Qualification: First 3 of each heat (Q) and the 6 fastest times (q) qualified for the semifinals.

Semifinals
Qualification: First 2 of each heat (Q) and the 2 fastest times (q) qualified for the final.