2016 Internazionali di Tennis dell'Umbria – Singles

Aljaž Bedene was the two-time defending champion but chose not to participate.

Miljan Zekić won the title after defeating Stefano Napolitano 6–7(6–8), 6–4, 6–3 in the final.

Seeds
• # 🇸🇰 Andrej Martin (quarterfinals)

• # 🇮🇹 Marco Cecchinato (semifinals)

• # 🇦🇷 Marco Trungelliti (semifinals)

• # 🇭🇺 Márton Fucsovics (quarterfinals)

• # 🇮🇹 Alessandro Giannessi (quarterfinals)

• # 🇮🇹 Luca Vanni (quarterfinals)

• # 🇦🇷 Facundo Argüello (first round)

• # 🇹🇷 Marsel İlhan (first round)