2016 Italian Open – Women's singles

Serena Williams defeated Madison Keys in the final, 7–6(7–5), 6–3 to win the women's singles tennis title at the 2016 Italian Open. This was Williams's second title in Rome won without dropping a set, and her fourth Italian Open title overall.

Maria Sharapova was the reigning champion, but could not defend her title because of her provisional suspension after failing a drug test at the Australian Open.

Seeds
The top eight seeds received a bye into the second round. • 🇺🇸 Serena Williams (champion)

• 🇩🇪 Angelique Kerber (second round)

• 🇪🇸 Garbiñe Muguruza (semifinals)

• 🇧🇾 Victoria Azarenka (second round)

• 🇨🇿 Petra Kvitová (second round)

• 🇷🇴 Simona Halep (second round)

• 🇮🇹 Roberta Vinci (second round)

• 🇪🇸 Carla Suárez Navarro (third round)

• 🇷🇺 Svetlana Kuznetsova (quarterfinals)

• 🇨🇿 Lucie Šafářová (second round)

• Timea Bacsinszky (quarterfinals)

• 🇺🇸 Venus Williams (second round)

• 🇷🇸 Ana Ivanovic (second round)

• 🇮🇹 Sara Errani (first round)

• 🇺🇦 Elina Svitolina (first round)

• 🇨🇿 Karolína Plíšková (first round)

Seeds
• # 🇰🇿 Yulia Putintseva (first round)

• # 🇷🇺 Elena Vesnina (qualifying competition)

• # 🇬🇧 Heather Watson (qualified)

• # 🇩🇪 Julia Görges (qualified)

• # 🇺🇸 Christina McHale (qualified)

• # Monica Puig (qualified)

• # 🇸🇪 Johanna Larsson (qualified)

• # 🇺🇸 Varvara Lepchenko (qualifying competition)

• # 🇺🇸 Irina Falconi (first round)

• # 🇺🇸 Madison Brengle (first round)

• # 🇨🇳 Zheng Saisai (first round)

• # 🇺🇸 Nicole Gibbs (first round)

• # Mirjana Lučić-Baroni (first round)

• # Hsieh Su-wei (qualifying competition)

• # 🇪🇸 Lara Arruabarrena (qualifying competition)

• # Ana Konjuh (qualifying competition)

Qualifiers
• # 🇨🇴 Mariana Duque Mariño

• # 🇺🇸 Alison Riske

• # 🇬🇧 Heather Watson

• # 🇩🇪 Julia Görges

• # 🇺🇸 Christina McHale

• # Monica Puig

• # 🇸🇪 Johanna Larsson

• # Kiki Bertens