2016 Japan Women's Open – Singles

Yanina Wickmayer was the defending champion, but lost in the first round to Viktorija Golubic.

Christina McHale won her first WTA title, defeating Kateřina Siniaková in the final, 3–6, 6–4, 6–4.

Seeds
• #🇯🇵 Misaki Doi (first round)

• #🇧🇪 Yanina Wickmayer (first round)

• #🇰🇿 Yulia Putintseva (second round)

• #🇸🇪 Johanna Larsson (second round)

• #🇺🇸 Madison Brengle (first round)

• #🇨🇳 Zhang Shuai (semifinals)

• #🇺🇸 Christina McHale (champion)

• #🇺🇦 Kateryna Bondarenko (first round)

Seeds
• #🇦🇹 Tamira Paszek (moved to main draw)

• #🇸🇰 Jana Čepelová (moved to main draw)

• #🇺🇸 Jennifer Brady (first round)

• #🇸🇪 Rebecca Peterson (qualified)

• #🇪🇸 Sara Sorribes Tormo (first round)

• #🇺🇸 Grace Min (withdrew, still playing in Dalian)

• #🇮🇱 Julia Glushko (first round)

• #🇹🇭 Luksika Kumkhum (first round)

• #🇷🇸 Aleksandra Krunić (withdrew)

• #🇯🇵 Hiroko Kuwata (first round)

• #🇰🇷 Jang Su-jeong (qualified)

• #🇩🇪 Antonia Lottner (qualifying competition, lucky loser)

Qualifiers
• #🇯🇵 Miyu Kato

• #🇰🇷 Jang Su-jeong

• #🇯🇵 Erika Sema

• #🇸🇪 Rebecca Peterson

Lucky losers

 * 1) 🇩🇪 Antonia Lottner