2016 Kentucky Bank Tennis Championships – Men's singles

John Millman was the defending champion but chose not to defend his title.

Ernesto Escobedo won the title after defeating Frances Tiafoe 6–2, 6–7(6–8), 7–6(7–3) in the final.

Seeds
• # 🇫🇷 Adrian Mannarino (second round)

• # 🇹🇳 Malek Jaziri (second round)

• # 🇬🇧 Daniel Evans (first round)

• # 🇦🇺 Jordan Thompson (second round)

• # 🇺🇸 Bjorn Fratangelo (first round)

• # 🇫🇷 Quentin Halys (first round)

• # 🇺🇸 Frances Tiafoe (final)

• # 🇺🇸 Stefan Kozlov (second round)