2016 Knoxville Challenger – Singles

Daniel Evans was the defending champion but lost in the second round to Peter Polansky.

Michael Mmoh won the title after defeating Polansky 7–5, 2–6, 6–1 in the final.

Seeds
• # 🇬🇧 Daniel Evans (second round)

• # 🇺🇸 Frances Tiafoe (second round)

• # 🇺🇸 Jared Donaldson (semifinals)

• # 🇺🇸 Bjorn Fratangelo (first round)

• # 🇺🇸 Denis Kudla (first round)

• # 🇺🇸 Tim Smyczek (second round)

• # Henri Laaksonen (first round)

• # 🇺🇸 Stefan Kozlov (semifinals)