2016 Mutua Madrid Open – Women's singles

Simona Halep defeated Dominika Cibulková in the final, 6–2, 6–4 to win the women's singles tennis title at the 2016 Madrid Open.

Petra Kvitová was the defending champion, but lost in the third round to Daria Gavrilova.

Seeds
• 🇵🇱 Agnieszka Radwańska (first round)

• 🇩🇪 Angelique Kerber (first round)

• 🇪🇸 Garbiñe Muguruza (second round)

• 🇧🇾 Victoria Azarenka (third round, withdrew)

• 🇨🇿 Petra Kvitová (third round)

• 🇷🇴 Simona Halep (champion)

• 🇮🇹 Roberta Vinci (first round)

• 🇪🇸 Carla Suárez Navarro (third round)

• 🇷🇺 Svetlana Kuznetsova (first round)

• Timea Bacsinszky (third round)

• 🇨🇿 Lucie Šafářová (second round, withdrew)

• 🇺🇦 Elina Svitolina (second round)

• 🇨🇿 Karolína Plíšková (second round)

• 🇷🇸 Ana Ivanovic (second round)

• 🇮🇹 Sara Errani (first round)

• 🇺🇸 Sloane Stephens (second round)

Seeds
• # 🇩🇪 Laura Siegemund (qualified)

• # 🇷🇺 Elena Vesnina (qualified)

• # 🇬🇧 Heather Watson (qualifying competition, lucky loser)

• # 🇩🇪 Julia Görges (first round)

• # Monica Puig (qualified)

• # 🇨🇳 Zheng Saisai (first round)

• # 🇺🇦 Kateryna Bondarenko (first round)

• # 🇺🇸 Irina Falconi (first round)

• # 🇺🇸 Madison Brengle (qualifying competition)

• # 🇺🇸 Nicole Gibbs (first round)

• # Mirjana Lučić-Baroni (qualified)

• # Ana Konjuh (first round)

• # 🇬🇧 Naomi Broady (qualifying competition)

• # 🇺🇸 Bethanie Mattek-Sands (qualifying competition)

• # 🇰🇿 Yaroslava Shvedova (first round)

• # 🇺🇸 Alison Riske (qualified)

Qualifiers
• # 🇩🇪 Laura Siegemund

• # 🇷🇺 Elena Vesnina

• # Mirjana Lučić-Baroni

• # 🇷🇴 Patricia Maria Țig

• # Monica Puig

• # 🇺🇸 Alison Riske

• # 🇨🇿 Kateřina Siniaková

• # 🇺🇸 Louisa Chirico

Lucky loser
• # 🇬🇧 Heather Watson