2016 Regional League Division 2 Central Region

 2016 Regional League Division 2 Central Region is the 1st season of the League competition since its establishment in 2016. It is in the third tier of the Thai football league system. The league winners and runners up will qualify for the 2016 Regional League Division 2 champions league round. This zone is broken from 2015 Thai Division 2 League Central & Eastern Region and 2015 Thai Division 2 League Central & Western Region

Break zone clubs

 * PTU Pathum Thani Seeker is broken from Central & Western Region
 * Saraburi TRU and Phan Thong are broken from Central & Eastern Region

Relegated clubs

 * Ayutthaya were relegated from the 2015 Thai Division 1 League.

Moved clubs

 * Chaiyaphum were moved from the North Eastern Region.
 * Nakhon Sawan, Singburi and Uthai Thani Forest were moved from the Northern Region.
 * Phichit were moved from the Northern Region.

Expansion clubs

 * Ayutthaya United Promoted from Khǒr Royal Cup (ถ้วย ข.) runner-up 2015
 * Ayutthaya Warrior authorize from  Phichit.

Renamed clubs

 * Kaeng Khoi TRU renamed Saraburi TRU
 * PTU Pathum Thani Seeker renamed PTU Pathum Thani

Returning clubs
Paknampho NSRU and Uthai Thani Forest are returning to the league after a 1-year break.

Top scorers

 * As of 3 September 2016.