2016 Rio Open – Men's singles

David Ferrer was the defending champion, but lost in the quarterfinals to Dominic Thiem.

Pablo Cuevas won the title, defeating Guido Pella in the final, 6–4, 6–7(5–7), 6–4.

Seeds
• #🇪🇸 Rafael Nadal (semifinals)

• #🇪🇸 David Ferrer (quarterfinals)

• #🇫🇷 Jo-Wilfried Tsonga (first round)

• #🇺🇸 John Isner (first round)

• #🇦🇹 Dominic Thiem (semifinals)

• #🇺🇸 Jack Sock (first round)

• #🇮🇹 Fabio Fognini (second round, retired)

• #🇧🇷 Thomaz Bellucci (first round)

Seeds
• # 🇫🇷 Paul-Henri Mathieu ''(qualifying competition, retired)

• # 🇯🇵 Taro Daniel (qualified)

• # 🇦🇷 Facundo Bagnis (qualified)

• # 🇧🇷 Rogério Dutra Silva (qualifying competition)

• # 🇪🇸 Daniel Gimeno-Traver (qualified)

• # 🇪🇸 Albert Montañés ''(qualifying competition, retired)

• # 🇦🇷 Facundo Argüello (qualifying competition)

• # Gastão Elias (qualified)

Qualifiers
• # 🇪🇸 Daniel Gimeno-Traver

• # 🇯🇵 Taro Daniel

• # 🇦🇷 Facundo Bagnis

• # Gastão Elias