2016 Rio Open – Women's singles

Sara Errani was the defending champion, but chose to compete in Dubai instead.

Francesca Schiavone won the title, defeating Shelby Rogers in the final, 2–6, 6–2, 6–2.

Seeds
• # 🇧🇷 Teliana Pereira (first round)

• # 🇸🇪 Johanna Larsson (second round)

• # 🇲🇪 Danka Kovinić (quarterfinals)

• # 🇺🇸 Christina McHale (first round)

• # Polona Hercog (second round)

• # 🇪🇸 Lara Arruabarrena (quarterfinals)

• # 🇩🇪 Tatjana Maria (first round)

• # 🇷🇴 Andreea Mitu (first round)

Seeds
• # 🇨🇴 Mariana Duque Mariño (qualified)

• # Maria Sakkari (qualifying competition)

• # 🇫🇷 Alizé Lim (qualifying competition)

• # 🇺🇸 Katerina Stewart (first round)

• # 🇪🇸 Laura Pous Tió (first round)

• # Cindy Burger (qualified)

• # 🇮🇱 Shahar Pe'er (first round)

• # 🇪🇸 Sara Sorribes Tormo (qualifying competition)

• # 🇱🇮 Stephanie Vogt (first round)

• # 🇦🇷 María Irigoyen (qualified)

• # 🇷🇴 Mihaela Buzărnescu (first round)

• # 🇺🇸 Jennifer Brady (qualified)

Qualifiers
• # 🇨🇴 Mariana Duque Mariño

• # 🇺🇸 Jennifer Brady

• # 🇧🇷 Paula Cristina Gonçalves

• # 🇧🇬 Elitsa Kostova

• # 🇦🇷 María Irigoyen

• # Cindy Burger