2016 São Paulo Challenger de Tênis – Singles

Guido Pella was the defending champion but chose not to participate.

Gonzalo Lama won the title, defeating Ernesto Escobedo 6–2, 6–2 in the final.

Seeds
• # 🇧🇷 André Ghem (first round)

• # 🇧🇷 João Souza (second round)

• # 🇧🇷 Guilherme Clezar (first round, retired)

• # 🇧🇷 Thiago Monteiro (semifinals)

• # Gonzalo Lama (champion)

• # Marcelo Arévalo (quarterfinals)

• # 🇸🇪 Christian Lindell (semifinals)

• # 🇦🇷 Maximiliano Estévez (second round, retired)