2016 Samarkand Challenger – Singles

Teymuraz Gabashvili was the defending champion, but chose not to defend his title.

Karen Khachanov won the title after defeating Rubén Ramírez Hidalgo 6–1, 6–7(6–8), 6–1 in the final.

Seeds
• # 🇷🇺 Karen Khachanov (champion)

• # 🇲🇩 Radu Albot (quarterfinals)

• # 🇮🇱 Amir Weintraub (quarterfinals)

• # 🇪🇸 Jordi Samper Montaña (second round)

• # 🇺🇿 Farrukh Dustov (first round, retired)

• # 🇰🇿 Dmitry Popko (second round)

• # 🇷🇸 Nikola Milojević (first round)

• # 🇪🇸 Enrique López Pérez (first round)