2016 Sparta Prague Open – Singles

Norbert Gombos was the defending champion but lost in the second round to Lukáš Rosol.

Adam Pavlásek won the title after defeating Stéphane Robert 6–4, 3–6, 6–3 in the final.

Seeds
• # 🇨🇿 Lukáš Rosol (quarterfinals)

• # 🇧🇷 Rogério Dutra Silva (quarterfinals)

• # 🇫🇷 Stéphane Robert (final)

• # 🇸🇰 Jozef Kovalík (semifinals)

• # 🇦🇹 Gerald Melzer (second round)

• # 🇨🇿 Adam Pavlásek (champion)

• # 🇸🇰 Andrej Martin (first round)

• # 🇪🇪 Jürgen Zopp (quarterfinals)