2016 St. Petersburg Ladies' Trophy – Singles

Jeļena Ostapenko was the defending champion, but lost in the first round to Yanina Wickmayer.

Second-seeded Roberta Vinci won the title, defeating Belinda Bencic in the final, 6–4, 6–3.

Both finalists entered the top 10 of WTA rankings for the first time at the conclusion of the tournament.

Seeds
The top four seeds received a bye into the second round. • # Belinda Bencic (final)

• # 🇮🇹 Roberta Vinci (champion)

• # Caroline Wozniacki (second round)

• # 🇷🇸 Ana Ivanovic (semifinals)

• # 🇷🇺 Anastasia Pavlyuchenkova (quarterfinals)

• # 🇸🇰 Anna Karolína Schmiedlová (withdrew due to an ankle sprain)

• # 🇫🇷 Kristina Mladenovic (first round)

• # 🇫🇷 Alizé Cornet (withdrew due to a lower back injury)

• # 🇷🇴 Monica Niculescu (second round)

Seeds
• # 🇩🇪 Laura Siegemund (qualifying competition, lucky loser)

• # 🇨🇿 Kateřina Siniaková (qualified)

• # 🇨🇿 Klára Koukalová (qualified)

• # 🇧🇬 Sesil Karatantcheva (qualifying competition)

• # 🇷🇴 Patricia Maria Tig (qualifying competition, lucky loser)

• # 🇦🇹 Tamira Paszek (qualified)

• # 🇺🇦 Maryna Zanevska (second round)

• # 🇯🇵 Misa Eguchi (first round)

Qualifiers
• # 🇺🇦 Kateryna Kozlova

• # 🇨🇿 Kateřina Siniaková

• # 🇨🇿 Klára Koukalová

• # 🇦🇹 Tamira Paszek

Lucky losers
• # 🇩🇪 Laura Siegemund

• # 🇷🇴 Patricia Maria Tig