2016 Torneo Internacional Challenger León – Singles

Austin Krajicek was the defending champion, but lost in the first round to Adrián Menéndez Maceiras.

Michael Berrer won the title, defeating João Souza 6–3, 6–2 in the final.

Seeds
• # 🇦🇺 Sam Groth (first round)

• # 🇺🇸 Taylor Fritz (first round)

• # 🇹🇳 Malek Jaziri (quarterfinals, retired)

• # 🇯🇵 Yūichi Sugita (first round)

• # 🇺🇸 Austin Krajicek (first round)

• # 🇩🇪 Michael Berrer (champion)

• # 🇫🇷 Stéphane Robert (first round)

• # 🇯🇵 Tatsuma Ito (first round)