2016 US Open – Girls' singles

Dalma Gálfi was the defending champion, but was no longer eligible to compete.

Kayla Day won the title, defeating Viktória Kužmová in the final, 6–3, 6–2.

Seeds
• # 🇷🇺 Anastasia Potapova (quarterfinals)

• # 🇷🇺 Olesya Pervushina (second round)

• # Rebeka Masarova (withdrew)

• # 🇺🇸 Amanda Anisimova (third round)

• # 🇺🇸 Kayla Day (champion)

• # 🇷🇺 Amina Anshba (first round)

• # 🇨🇦 Bianca Andreescu (semifinals)

• # 🇺🇸 Sofia Kenin (semifinals)

• # 🇺🇸 Usue Maitane Arconada (third round)

• # 🇺🇸 Claire Liu (second round)

• # 🇷🇸 Olga Danilović (first round, retired)

• # 🇺🇦 Katarina Zavatska (first round)

• # 🇸🇰 Viktória Kužmová (final)

• # Kaja Juvan (first round)

• # 🇯🇵 Yuki Naito (third round)

• # 🇺🇸 Alexandra Sanford (third round)

Seeds
• # 🇷🇺 Marta Paigina (qualified)

• # 🇨🇳 Du Zhima (qualifying competition)

• # Cho I-hsuan (qualifying competition)

• # 🇮🇹 Federica Bilardo (withdrew)

• # 🇨🇳 Cao Siqi (first round)

• # 🇺🇸 Ellie Douglas (first round)

• # 🇸🇪 Ida Jarlskog (first round)

• # 🇮🇹 Ludmilla Samsonova (first round)

• # 🇺🇸 Kylie McKenzie (qualified)

• # 🇺🇸 Ashley Lahey (qualified)

• # 🇯🇵 Mayuka Aikawa (first round)

• # 🇮🇳 Pranjala Yadlapalli (qualified)

• # 🇮🇱 Shelly Krolitzky (qualified)

• # 🇩🇪 Irina Cantos Siemers (qualified)

• # 🇮🇹 Lucrezia Stefanini (qualifying competition)

• # 🇺🇸 Hurricane Tyra Black (first round)

Qualifiers
• # 🇷🇺 Marta Paigina

• # 🇺🇸 Ashley Lahey

• # 🇺🇸 Kylie McKenzie

• # 🇮🇳 Pranjala Yadlapalli

• # 🇮🇱 Shelly Krolitzky

• # 🇩🇪 Irina Cantos Siemers

• # 🇺🇸 Hailey Baptiste

• # 🇵🇱 Daria Kuczer

Lucky loser
• # 🇺🇸 Vanessa Ong