2016 UniCredit Czech Open – Singles

Jiří Veselý was the defending champion, but lost in the quarterfinals to eventual champion Mikhail Kukushkin.

Kukushkin won the title, defeating Márton Fucsovics in the final, 6–1, 6–2.

Seeds
• # 🇱🇹 Ričardas Berankis (quarterfinals)

• # 🇨🇿 Lukáš Rosol (quarterfinals)

• # 🇨🇿 Jiří Veselý (quarterfinals)

• # 🇪🇸 Íñigo Cervantes (semifinals)

• # 🇧🇦 Damir Džumhur (first round, retired)

• # 🇰🇿 Mikhail Kukushkin (champion)

• # 🇧🇷 Rogério Dutra Silva (second round)

• # Robin Haase (first round)