2016 Western & Southern Open – Men's singles

Marin Čilić defeated Andy Murray in the final, 6–4, 7–5 to win the men's singles tennis title at the 2016 Cincinnati Masters. It was his maiden Masters 1000 title, ending a streak of 18 consecutive Masters events won by a member of the Big Four.

Roger Federer was the two-time reigning champion, but withdrew due to a knee injury.

Seeds
The top eight seeds receive a bye into the second round. • 🇬🇧 Andy Murray (final)

• Stan Wawrinka (third round)

• 🇪🇸 Rafael Nadal (third round)

• 🇨🇦 Milos Raonic (semifinal)

• 🇯🇵 Kei Nishikori (third round)

• 🇨🇿 Tomáš Berdych (third round)

• 🇫🇷 Jo-Wilfried Tsonga (third round)

• 🇦🇹 Dominic Thiem (quarterfinals)

• 🇫🇷 Gaël Monfils (third round, withdrew due to back injury)

• 🇪🇸 David Ferrer (first round)

• 🇧🇪 David Goffin (second round)

• Marin Čilić (champion)

• 🇫🇷 Richard Gasquet (second round)

• 🇦🇺 Nick Kyrgios (second round)

• 🇪🇸 Roberto Bautista Agut (first round)

• 🇪🇸 Feliciano López (second round)

Seeds
• # 🇨🇿 Jiří Veselý (qualified)

• # 🇦🇷 Guido Pella (first round)

• # 🇷🇺 Mikhail Youzhny (qualified)

• # 🇫🇷 Stéphane Robert (first round)

• # 🇹🇳 Malek Jaziri (qualified)

• # Gastão Elias (qualifying competition)

• # 🇺🇦 Illya Marchenko (first round)

• # Lu Yen-hsun (first round)

• # 🇫🇷 Adrian Mannarino (qualifying competition, lucky loser)

• # 🇦🇷 Diego Schwartzman (first round)

• # 🇫🇷 Paul-Henri Mathieu (first round)

• # 🇦🇺 John Millman (qualified)

• # 🇧🇦 Damir Džumhur (first round)

• # Ivan Dodig (first round)

Qualifiers
• # 🇨🇿 Jiří Veselý

• # 🇯🇵 Yūichi Sugita

• # 🇷🇺 Mikhail Youzhny

• # 🇦🇺 John Millman

• # 🇹🇳 Malek Jaziri

• # Nikoloz Basilashvili

• # 🇩🇪 Mischa Zverev

Lucky losers
• # 🇫🇷 Adrian Mannarino