2016 Winnipeg National Bank Challenger – Women's singles

Kristie Ahn was the defending champion, but decided not to participate this year.

Francesca Di Lorenzo won the title, defeating Erin Routliffe 6–4, 6–1 in the final.

Seeds
• #🇮🇱 Julia Glushko (first round)

• #🇷🇺 Ksenia Lykina (first round)

• #🇺🇸 Lauren Albanese (second round)

• #🇨🇦 Carol Zhao (second round)

• #🇦🇺 Alison Bai (first round)

• #🇺🇸 Ellie Halbauer (second round)

• #🇫🇷 Caroline Roméo (first round)

• # Hsu Chieh-yu (first round)